chromosomal DNA from a Paraguayan Trypanosoma cruzi isolate was extracted and sonicated. The fragments were cloned and some clones were size-selectd and sequenced. Two primer pairs TC22 and TC4 were synthesized and applied to other Paraguayan T. cruzi isolates and two Leishmania mexicana amazoniensis str...
The polymerase chain reaction (PCR) was usedto evaluate the course of treatment of an acutely infected patient with Trypanosoma cruzi. The reactions were performed using a primer pair (TCZ1 and TCZ2) which prime the amplification of a highly repetive 195-bp T. cruzi genomic DNA. Treatment with benzonidaz...
Anatomopathological studies were performed in 15 placentas of pregnant women who were seropositive for Chagas' disease. Nests of Trypanosoma cruzi amastigote s were found in the placenta and umbilical cord of three of them (20 percent). The parasitism was extensive in two of the cases, where the nests we...